Dr. John Campbell: Cannabidiol and COVID

Dr. John Campbell: Cannabidiol and COVID Dr. John Campbell: Cannabidiol and COVID
Don Brady Photo

Don Brady
3 weeks 0 Views

Cannabidiol Inhibits SARS-CoV-2 Replication and Promotes the Host Innate Immune Response

Cannabidiol (CBD)

Potential regulator of the host stress and antiviral inflammatory responses

(cannabinoid class of natural products)

Hemp, cannabis plants,
Less than 0.3% psychotropic tetrahydrocannabinol (THC)

Oral solution of CBD is a U.S. FDA approved drug

High-purity CBD inhibits SARS-CoV-2 replication in human lung epithelial cells

Pretreated human lung cells (with ACE2 receptor)

for 2 hours with CBD before infection with SARS-CoV-2.

CBD potently inhibited viral replication under nontoxic conditions

(no toxicity was observed at the effective doses)

Worked in other human lung cell lines, worked in monkey kidney cells

Tested on Wuhan strain and 3 VOCs

Their ability to infect cells was comparably inhibited by CBD

CBD or other cannabinoids?

Checked 97 to 100% pure using quantitative nuclear magnetic resonance (qNMR).

No toxicity was observed for any of the CBD preparations at the doses used to inhibit viral infection

The CBD metabolite 7-OH-CBD, 7-hydroxy-cannabidiol

exhibits antiviral activity

Others did not work

Combining CBD with THC (1:1) significantly suppressed CBD efficacy,

consistent with competitive inhibition by THC.

CBD and 7-OH-CBD metabolite are the active and equipotent ingredients for the treatment of epilepsy

CBD acts at an early step after viral entry into cells

CBD targets host cell processes.

CBD inhibits viral RNA expression and reverses viral-induced changes in host gene expression

CBD effectively eradicated viral RNA expression in the host cells

Prevents changes in the cell produced by the virus to force the cell to produce new viral particles

CBD induces strong activation of genes associated with the host stress response.

Together, these results suggest that CBD acts to prevent viral protein translation and associated cellular changes.

Induction of viral genes for spike, envelope, and nucleocapsid proteins is reduced ~99% with CBD

CBD induction of increased transcription of 3 defensive endoplasmic reticulum genes

CBD induces interferon expression as part of its antiviral activity

CBD suppress viral infection,

and promote degradation of viral RNA

(SARS-CoV-2 infection suppresses the interferon signaling pathway)

CBD sufficiently lowers the effective viral titer to enable normal host activation of the interferon pathway.

CBD effectively reversed viral induction of cytokines that can lead to deadly cytokine storm

CBD treatment significantly inhibits SARS-CoV-2 replication in mice

After viral the challenge,

administration of CBD continued twice daily for an additional 4 days

CBD treatment significantly inhibited viral replication in lungs and nasal turbinates at day 5 after infection

Lower dose of CBD reduced viral load by 4.8-fold in lungs

Higher dose of CBD reduced viral load by 40 -fold in lungs

During this period, the mice showed no signs of clinical disease

These results establish the preclinical efficacy of CBD as an antiviral drug for SARS-CoV-2 during early stages of infection.

CBD is negatively associated with indications of SARS-CoV-2 infection in patient medical records

Given that high-purity CBD preparations are taken by a large number of individuals,

we examined whether CBD prescriptions are associated with indications of SARS-CoV-2 infection

An oral solution of CBD (100 mg/ml) (CBD100) is often used for the treatment of seizures

Analysis of 1,212 patients

Matched to control group counterparts

6.2% (75 patients) with CDB

8.9% for non-CBD patients

(P = 0.014)

Odds ratio (OR) of 0.65, (P = 0.009)

Odds ratio (OR) of 0.48, (P = 0.006)

Potential of Cannabidiol for the Treatment of Viral Hepatitis
https://scholar.google.com/scholar_lookup?title=Potential +of+cannabidiol+for+the+treatment+of+viral+hepatitis&author=H.+I.+Lowe&author=N.+J.+Toyang&author=W.+McLaughlin&publication_year=2017&journal=Pharm.+Res.&pages=116-118

CBD inhibited HCV replication by 86.4%

(Not Hep B)

Other RNA viral infections

Common cold




Hep C and E